376 papers
This study utilizes cryo-EM structures of phosphorylated MEK1 bound to ERK1 to reveal a novel, unexpected phosphorylation mechanism involving phosphate transfer from ERK1 Y204 to T202, alongside MEK1's phosphatase activity, thereby elucidating the complete catalytic cycle of this critical oncogenic pathway.
Using brain organoids and multi-omic analyses, this study reveals that the maternal allele bias of the imprinted gene *Copg2* during neurogenesis is established by an enhancer-driven activation in neural progenitors and subsequently maintained by *MestXL*-mediated repression of the paternal allele in neurons, rather than being solely initiated by transcriptional interference.
By combining Laser Capture Microdissection with RNA sequencing, this study reveals distinct, location-specific gene expression profiles in oral and aboral tissues of the coral *Pocillopora acuta*, highlighting unique functional specializations in environmental interaction and skeleton formation while demonstrating that many specialized functions rely on multi-gene patterns rather than single biomarkers.
This study integrates transcriptomics and proteomics to generate a high-resolution spatial map of cilia-associated proteins in the human fallopian tube, revealing their subcellular localization and providing new insights into the molecular mechanisms underlying infertility and reproductive disorders.
This study demonstrates a robust precision breeding strategy using CRISPR/Cas9, base editing, and prime editing to successfully integrate semi-dwarfing alleles into diverse Watkins wheat landraces, thereby unlocking their valuable genetic diversity for sustainable crop improvement.
By integrating single-cell RNA sequencing with multiplex immunohistochemistry, this study establishes a scalable framework for spatially mapping human spermatogenesis, revealing critical mRNA-to-protein expression discordances such as the delayed abundance of PIWIL4 protein relative to its transcripts.
This study identifies the ORFan gene 5.4 as encoding the essential spike tip protein of bacteriophage T4, which is required for infecting bacteria with truncated lipopolysaccharides despite being dispensable for viral particle assembly.
This study reveals that sequential waves of translation, orchestrated by the interplay of Ewsr1b, Syncrip, and HuR proteins and regulated by alternative 3'UTR lengths, coordinate the precise timing and localization of protein synthesis to drive embryonic development.
This study introduces a high-throughput microfluidic assay to systematically characterize gametogenic rejuvenation in *S. cerevisiae*, revealing that selective autophagy of the endoplasmic reticulum mediated by Atg39 and Atg40 is essential for resetting replicative lifespan.
This study systematically profiles the responses of isogenic BAF complex knockout cells to diverse genotoxic and cytotoxic agents, revealing distinct subtype-specific vulnerabilities, synthetic lethal interactions with MEK and EGFR inhibitors, and limited functional redundancy among ARID paralogs in genome stability and cell cycle regulation.
This study reveals that the Top3-Sgs1 complex coordinates the transcription-coupled migration of Holliday junctions from Spo11-induced break sites to cohesin-associated regions of convergent transcription, where crossovers are preferentially resolved to ensure accurate meiotic chromosome segregation.
This study utilizes cryo-electron microscopy and functional assays to reveal that IWS1 acts as a modular scaffold for the RNA polymerase II transcription elongation complex, employing its intrinsically disordered C-terminal region to coordinate multiple interactions that drive recruitment, stimulate elongation, and protect the complex from inhibition by RECQL5.
Although autism-associated M65I and S97L mutations in the Homer1 EVH1 domain do not significantly alter its overall structure or binding affinity, they perturb the domain's internal dynamics on the microsecond-to-millisecond timescale, with the M65I variant additionally exhibiting thermal destabilization.
This study reveals that neural microexons in both humans and chickens rely on conserved, accessible RNA secondary structures within the branchpoint-to-3' splice site region to facilitate spliceosome assembly and regulate stage-specific splicing during neurodevelopment.
This study reveals that orphan yeast septins are stabilized against degradation through two parallel mechanisms: the formation of transient coiled-coil homo-oligomers via their C-terminal domains and the action of the disaggregase chaperone Hsp104, ensuring stoichiometric fidelity despite variable mRNA levels.
This study presents a dual-channel microfluidic platform featuring organoid-derived endometrial cells that successfully replicates key physiological hallmarks of human implantation, enabling the observation of embryo adhesion, lineage segregation, and functional trophoblast activity.
This study reveals that the garden dormouse cerebral cortex undergoes stage-specific transcriptional reprogramming during hibernation, characterized by extensive gene regulation during torpor progression and a rapid, coordinated reversal of these metabolic and proteostatic programs during early arousal to preserve neural integrity.
This study demonstrates that the PCR-free Nicking Loop™ targeted library preparation method is fully compatible with MGI's DNBSEQ™ sequencing platform, achieving performance metrics and variant detection accuracy comparable to its established use on Illumina systems.
This paper elucidates the molecular mechanism by which coilin, the scaffolding protein of Cajal bodies, discriminates between mature and immature snRNPs through a bipartite C-terminal module comprising an RG-repeat RNA-binding region and a Tudor-like domain that specifically binds Sm proteins, thereby facilitating the sequestration of defective complexes.
This study reveals that the tRNA-modifying enzyme ELP3 sustains prostate cancer cell proliferation by regulating the expression of specific proteins through six distinct di-codon motifs (E3dDCs) and their local sequence contexts, rather than through simple codon frequency, thereby linking tRNA modifications to proteome composition and mitotic fidelity.